The effect of isolated phosphorus solubilizing bacteria and molasses on Soluble and available P in wheat straw medium

Document Type: Original Article

Authors

1 M.Sc of Soil science, University of Yasouj

2 Associate professorof Soil science, University of Yasouj, Iran

3 Assistant professor of Microbiology,University of Yasouj, Iran

4 Associate professor of Soil science, University of Yasouj, Iran

Abstract

Phosphorus solubilizing microorganisms are capable of transforming insoluble phosphorus (P) forms to soluble and bioavailable P. The aim of the present study was to isolate P solubilizing bacteria from forest soils around the rock phosphate (RP) mine of Koh-e-SepidLar in Kohgiluyeh and Boyer Ahmad province and evaluate their ability in Sperber culture medium and wheat straw medium in the presence of RP and molasses. Bacteria were isolated and conducted by serial dilution and culturedon Pikovskaya medium. Quantitative assessment of 6 isolated bacteria carried out in the Sperber medium with tricalcium phosphate with 3 replications. Soluble P was measured on 5, 10, 15 and 20 days. The ability of two most effective bacteria in the liquid culture, assessed in wheat straw medium in a completely randomized design. Treatments consisted of a factorial combination of three RP levels (zero, 1:8 and 1:4 RP: wheat straw) and molasses in three rates (zero, 0.5 and 1% w/w) and three bacteria levels (none inoculated, and inoculated with isolates 1 and 2, separately). Soluble P and available P were measured on 20 and 40 days with CaCl2 0.01 M and NaHCO3 0.5 M, respectively. Some bacteria with P solubilizing capability found in the Pikovskaya medium. Two isolates showed the highest ability in liquid culture. CaCl2-P in the presence of isolates 1 and 2 was higher than blank in wheat straw medium. Molasses caused the increase of CaCl2-P in both times. Presence of RP reduced CaCl2-P.
Highlights

Isolation of P solubilizing bacteria from forest soils around the rock phosphate (RP) mine in Kohgiluyeh and Boyer Ahmad
Molasses caused the increase of CaCl2-P in both time.
Presence of RP reduced CaCl2-P.

Keywords


Article Title [Persian]

The effect of isolated phosphorus solubilizing bacteria and molasses on Soluble and available P in wheat straw medium

Authors [Persian]

  • کبری شیخی 1
  • ابراهیم ادهمی 2
  • رضا نقی ها 3
  • حمیدرضا اولیایی 4
1 دانشگاه یاسوج، ایران
2 دانشگاه یاسوج، ایران
3 دانشگاه یاسوج، ایران
4 دانشگاه یاسوج، ایران
Abstract [Persian]

Phosphorus solubilizing microorganisms are capable of transforming insoluble phosphorus (P) forms to soluble and bioavailable P. The aim of the present study was to isolate P solubilizing bacteria from forest soils around the rock phosphate (RP) mine of Koh-e-SepidLar in Kohgiluyeh and Boyer Ahmad province and evaluate their ability in Sperber culture medium and wheat straw medium in the presence of RP and molasses. Bacteria were isolated and conducted by serial dilution and culturedon Pikovskaya medium. Quantitative assessment of 6 isolated bacteria carried out in the Sperber medium with tricalcium phosphate with 3 replications. Soluble P was measured on 5, 10, 15 and 20 days. The ability of two most effective bacteria in the liquid culture, assessed in wheat straw medium in a completely randomized design. Treatments consisted of a factorial combination of three RP levels (zero, 1:8 and 1:4 RP: wheat straw) and molasses in three rates (zero, 0.5 and 1% w/w) and three bacteria levels (none inoculated, and inoculated with isolates 1 and 2, separately). Soluble P and available P were measured on 20 and 40 days with CaCl2 0.01 M and NaHCO3 0.5 M, respectively. Some bacteria with P solubilizing capability found in the Pikovskaya medium. Two isolates showed the highest ability in liquid culture. CaCl2-P in the presence of isolates 1 and 2 was higher than blank in wheat straw medium. Molasses caused the increase of CaCl2-P in both times. Presence of RP reduced CaCl2-P.
Highlights

Isolation of P solubilizing bacteria from forest soils around the rock phosphate (RP) mine in Kohgiluyeh and Boyer Ahmad
Molasses caused the increase of CaCl2-P in both time.
Presence of RP reduced CaCl2-P.

Keywords [Persian]

  • Phosphate solubilizing bacteria
  • Rock phosphate
  • Molasses
(1)  Hsanzadeh, E., Mazaheri, D., ChayeiChy, M.R., Khavazy, K. To facilitate the absorption of phosphorus and bacteria use efficiency of fertilizer phosphorus on yield and yield components of barley. Research and development in agriculture and Gardening 2007, 77, pp 111-118.

(2)    Khavazy, K., Malakoti, M.J. The need for industrial production of biofertilizers in Iran, Agricultural Research, Education and Extension Organization Press, Tehran, 2001.

(3)  Sing, H., Reddy, M.S. Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. European Journal of Soil Biology 2010, 47, pp 30-34.

(4)  Darzi, M.T.,Ghlavnd A.,Rejali F. Effect of mycorrhiza, vermicompost and phosphate fertilizer on flowering biology, biological yield and root symbiosis in fennel. Journal of Agricultural Science2008, 10, pp 88-109.

(5)  Pyghami, Ashenaee S. Influence of culture media on antagonistic efficacy of some strain of Pseudomonas fluorescens and Bacillus subtilis. MSC thesis. University of Tehran, Iran. 2007 pp 12-30.

(6)  Jahangirzadeh N., Adhami E., Flahati S. The effect of nitrogen and phosphate soil Malasses in the context of the dissolution of wheat straw by fungi phosphate solubilizing, eighth biotechnology conference in Tehran 2012.

(7)  Shojaalsadati A. Industerial biotechnology. University of tarbiatmoddares, 2003, pp 43-45.

(8)  Bouyoucou G. J. Hydrometer method improved for making particle size analysis of soil. Agronomy Journal 1962, 54, pp 464-465.

(9)  Nelson D. W., Sommers. L. E. Total carbon, organic carbon, andorganic matter.In Methods of soil analysis 1996, pp 961–1010.

(10)             Leoppert R. H. Suarrez. D. L. Organic and Inorganic Forms of Phosphorus in a Calcareous Soil Methods of soil analysis 1996, pp 437–474.

(11)             Chapman HD. Cationexchang capacity. PP. 891-900. In: C. A. Black (Eds.), Methods of soil Analysis. Part II. American Society of Agronomy, Madison, WI 1965, Vol. 22, pp 230-239.

(12)             Murphy J., Riley. I.P.A modified single solution method for the determination of phosphate in natural waters. Analysis Chimistry 1962, 27, pp 31-36.

(13)             Vazquez F., Holguim G., Puente M. E. Lopez A. and Bashar Y. Phosphate sioubilizing microorganisms associated with the rhizosphere of mogroves in a semiarid castallogoan.Biology and Fertility of Soils2000¸ 30, pp 460-468.

(14)             Kucey R. M. N. Phosphate solubilizing bacteria and fungi in various cultivated and virgin alberta soils. Canadian journal of Soil Science 1983, 63, pp 671-678.

(15)             Nehas E. Factors affecting the solubilization of insoluble phosphates, Abstract Book first International meeting on microbial phosphate solubilization 2002, pp16-19.

(16)             Redriguez H., Fraga R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 1999, 17, pp 319-339.

(17)             Esmaeelkho N., Ali- khani A. The effect of phosphate solubilizing bacteria as organic fertilizer on the environment, the third national congress of fuel, energy and the environment 2013.

(18)             Chen Y.P., Rekha P.D., Arun A.B., Shen F.T., Lai. W.A., Young C.C. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 2006, 34, pp 33-41.

(19)             Adhami E., Maftoun M., Ronaghi A., Karimian N.,Yasrebi J., Assad M. T. Inorganic Phosphorus fractionation of highly calcareous soils of Iran. Commun. Soil Science and Plant Analysis 2006, 37, pp 1877-1888.

(20)             Samadi A., Gilkes R. J. Phosphorus transformations and their relationships with calcareous soil properties of south Western Australia. Soil Science Society of American Journal 1999, 63, pp 809-815.

(21)             Jorizi M. Assessment of raw phosphate rock dissolution by phosphate solubilizing fungi. End-a master, Yasouj University, College of Agriculture 2012.

(22)             Sahu S.N., Jana B.B. Enhancement of fertilizer value of oak phosphate engineered through phosphate solubilization bacteria. Ecological Engineering 1997, 15, pp 27-39.

(23)             Gyaneshwar P., Kumar G.N. Parekh L.J., Poole P.S. Role of soil microorganisms in improving p nutrition of plants. Plant and Soil 2002, 245, pp 83-93.